Invitrogen

EOMES Monoclonal Antibody (Dan11mag), PE-eFluor™ 610, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (15)

FIGURE: 1 / 16

EOMES Antibody (61-4875-80) in Flow

Surface staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) followed by intracellularly staining with 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse EOMES PE-eFluor® 610 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

61-4875-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Dan11mag

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574613

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric of mouse splenocytes analysis using Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Invasive margin tissue-resident macrophages of high CD163 expression impede responses to T cell-based immunotherapy.

Journal for immunotherapy of cancer

van Elsas MJ,Labrie C,Etzerodt A,Charoentong P,van Stigt Thans JJC,Van Hall T,van der Burg SH

61-4875-82 was used in Flow Cytometry to identify a small but discernible CD163hi macrophage population, with high expression of several tumor-promoting macrophage markers and a functional anti-inflammatory transcriptome profile, but not other macrophages, to be responsible.

Wed Mar 01 00:00:00 EST 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

The transcription factor HIF-1α mediates plasticity of NKp46+ innate lymphoid cells in the gut.

The Journal of experimental medicine

Krzywinska E,Sobecki M,Nagarajan S,Zacharjasz J,Tambuwala MM,Pelletier A,Cummins E,Gotthardt D,Fandrey J,Kerdiles YM,Peyssonnaux C,Taylor CT,Sexl V,Stockmann C

61-4875-82 was used in Flow cytometry/Cell sorting to suggest that HIF-1α shapes the ILC phenotype in the gut.

Mon Feb 07 00:00:00 EST 2022

Interleukin-1β secretion induced by mucosa-associated gut commensal bacteria promotes intestinal barrier repair.

Gut microbes

Wu WH,Kim M,Chang LC,Assie A,Saldana-Morales FB,Zegarra-Ruiz DF,Norwood K,Samuel BS,Diehl GE

61-4875-82 was used in Flow cytometry/Cell sorting to demonstrate an unexpected role for commensal bacteria in promoting IL-1β secretion to support intestinal barrier repair.

Thu Feb 24 00:00:00 EST 2022

PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity.

Cancer discovery

Wiede F,Lu KH,Du X,Zeissig MN,Xu R,Goh PK,Xirouchaki CE,Hogarth SJ,Greatorex S,Sek K,Daly RJ,Beavis PA,Darcy PK,Tonks NK,Tiganis T

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

CD8⁺ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis.

Cell death & disease

Feizi N,Focaccetti C,Pacella I,Tucci G,Rossi A,Costanza M,Pedotti R,Sidney J,Sette A,La Rocca C,Procaccini C,Matarese G,Barnaba V,Piconese S

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Innate and adaptive immune responses following PD-L1 blockade in treating chronic murine alveolar echinococcosis.

Parasite immunology

Jebbawi F,Bellanger AP,Lunström-Stadelmann B,Rufener R,Dosch M,Goepfert C,Gottstein B,Millon L,Grandgirard D,Leib SL,Beldi G,Wang J

61-4875-82 was used in Flow cytometry/Cell sorting to suggest that the PD-L1 pathway plays an important role by regulating adaptive and innate immune cells against E. multilocularis infection, with significant modulation of tissue inflammation.

Sun Aug 01 00:00:00 EDT 2021

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation.

JCI insight

Hope HC,Brownlie RJ,Fife CM,Steele L,Lorger M,Salmond RJ

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies.

Cancer cell

Si J,Shi X,Sun S,Zou B,Li Y,An D,Lin X,Gao Y,Long F,Pang B,Liu X,Liu T,Chi W,Chen L,Dimitrov DS,Sun Y,Du X,Yin W,Gao G,Min J,Wei L,Liao X

61-4875-82 was used in Flow cytometry/Cell sorting to investigate if the HPK1-NFκB-Blimp1 axis mediates T cell dysfunction.

Mon Oct 12 00:00:00 EDT 2020

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

61-4875 was used in Flow cytometry/Cell sorting to provide further insights into vaccine-induced multifaceted mucosal T cell immunity with implications in the development of vaccines against respiratorypathogens, including influenza virus and SARS-CoV-2.

Tue Sep 22 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Tue Sep 22 00:00:00 EDT 2020

Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors.

Cell

Ponzetta A,Carriero R,Carnevale S,Barbagallo M,Molgora M,Perucchini C,Magrini E,Gianni F,Kunderfranco P,Polentarutti N,Pasqualini F,Di Marco S,Supino D,Peano C,Cananzi F,Colombo P,Pilotti S,Alomar SY,Bonavita E,Galdiero MR,Garlanda C,Mantovani A,Jaillon S

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Thu Jul 11 00:00:00 EDT 2019

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.

Nature communications

Istaces N,Splittgerber M,Lima Silva V,Nguyen M,Thomas S,Le A,Achouri Y,Calonne E,Defrance M,Fuks F,Goriely S,Azouz A

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Wed Jul 24 00:00:00 EDT 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

61-4875 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Mon May 21 00:00:00 EDT 2018

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

61-4875 was used in Flow cytometry/Cell sorting to examine the role of Tbet-dependent type I innate lymphoid cells in the murine liver.

Sat Jan 09 00:00:00 EST 2021

CXCR6 marks a novel subset of T-bet(lo)Eomes(hi) natural killer cells residing in human liver.

Scientific reports

Stegmann KA,Robertson F,Hansi N,Gill U,Pallant C,Christophides T,Pallett LJ,Peppa D,Dunn C,Fusai G,Male V,Davidson BR,Kennedy P,Maini MK

61-4875-82 was used in Flow cytometry/Cell sorting to identify surface expression of CXCR6 as a marker of a novel subset of T-bet(lo)Eomes(hi) natural killer cells residing in the human liver.

Mon May 23 00:00:00 EDT 2016

CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Qui HZ,Hagymasi AT,Bandyopadhyay S,St Rose MC,Ramanarasimhaiah R,Ménoret A,Mittler RS,Gordon SM,Reiner SL,Vella AT,Adler AJ

Published figure using EOMES monoclonal antibody (Product # 61-4875-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2011

IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions.

Journal of immunology (Baltimore, Md. : 1950)

Marshall HD,Prince AL,Berg LJ,Welsh RM

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

View more View less

Gene Aliases: C77258; Eomes; TBR-2; Tbr2

View more View less

UniProt ID: (Mouse) O54839

View more View less

Entrez Gene ID: (Mouse) 13813

View more View less

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

EOMES Monoclonal Antibody (Dan11mag), PE-eFluor™ 610, eBioscience™

EOMES Antibody (61-4875-80) in Flow

Product Details

61-4875-80

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!